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Alacalase‐Hydrolyzed Camellia Seed Hull Inhibited Angiotensin Converting Enzyme Activity and Lowered Blood Pressure in Spontaneous Hypertensive Rats
Author(s) -
Pan JeongHoon,
Lim HoJeong,
Shin EuiCheol,
Kim Jae Kyeom
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.793.9
Subject(s) - hydrolysate , hydrolysis , chemistry , blood pressure , camellia , angiotensin converting enzyme , renin–angiotensin system , enzyme , japonica , angiotensin ii , medicine , endocrinology , food science , pharmacology , biochemistry , biology , botany
High blood pressure is one of the major risk factors for various diseases and angiotensin‐converting enzyme (ACE) plays a critical role in the renin‐angiotensin‐aldesterone system; specifically, ACE can elevate blood pressure by converting decapeptide angiotensin I to oxtapeptide angiotensin II, a potent vasopressor. In our study, the responsive surface methodology was adopted to establish optimal Alcalase‐hydrolysis conditions of camellia seed hull against ACE activity. The optimum conditions are hydrolysis temperature of 50.98°C, Enzyme/Substrate ratio of 2.85%, and hydrolysis pH of 7.12. In an animal feeding study, SHRs were treated with either a low or high dose of hydrolysed C. japonica seed cake over 5 weeks. Even though SBP was not statistically different, the high dose of C. japonica hydrolysate lowered DBP (106±4.4 mmHg vs. 145±5.9 mmHg) at 5 th week. A similar trend was also observed in serum ACE activity. Lipid markers, (total plasma cholesterol, total plasma triacylglycerol, and plasma HDL cholesterol) were not influenced by the C. japonica hydrolysate. Considering that this camellia seed hull is a major resource of this plant, our study provides interesting preliminary data to utilize this plant for both academic and industrial applications. Support or Funding Information This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF‐2014R1A1A2058119).