Premium
Effects of Lycium Barbarum Polysaccharides Extract and Betaine on Cell Activation and Liver Fibrosis in Rat Hepatic Stellate HSC‐T6 Cells
Author(s) -
Chao Jane CJ,
Li BoYing
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.793.5
Subject(s) - hepatic stellate cell , lycium , betaine , chemistry , extracellular matrix , transforming growth factor , fibrosis , microbiology and biotechnology , biochemistry , endocrinology , medicine , biology , pathology , alternative medicine
The fibrogenesis is indicated by the activation of hepatic stellate cells (HSCs) and accompanied with enhanced secretion of transforming growth factor‐β (TGF‐β) and deposition of extracellular matrix. We determined the effects of Lycium barbarum polysaccharides (LBP) and betaine on cell activation and fibrotic markers after the activation of rat HSC‐T6 cells by H 2 O 2 . The results showed that H 2 O 2 at 5 μM activated HSC‐T6 cells by significantly increasing the expression of alpha‐smooth muscle actin (α‐SMA), TGF‐β, and matrix metalloproteinase (MMP)‐2, which were significantly reduced by LBP and betaine at 10 and/or 50 μg/mL (p<0.05). The expression of tissue inhibitor of metalloproteinase‐1 (TIMP‐1) was significantly inhibited by LBP at 10 μg/mL, however, the expression of TIMP‐2 was promoted by betaine at 10 μg/mL. Therefore, LBP and betaine, the active components in Lycium barbarum L., attenuate liver fibrosis through inhibiting cell activation of HSCs and expression of fibrotic markers under H 2 O 2 ‐induced oxidative stress. Support or Funding Information The study is supported by the Ministry of Science and Technology, Taiwan (grant no. 105‐2320‐B‐038‐036‐MY3).