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Consumption of whole aronia berry powder inhibits adoptive transfer‐induced colitis in mice
Author(s) -
Pei Ruisong,
Martin Derek,
Bolling Bradley
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.793.21
Subject(s) - colitis , adoptive cell transfer , lamina propria , inflammation , immunology , lymph , chemistry , mesenteric lymph nodes , weight gain , endocrinology , spleen , medicine , t cell , andrology , immune system , biology , body weight , pathology , epithelium
Background Aronia berries are rich in polyphenols which may inhibit inflammation by modulating gut immunocyte function. However, the mechanisms responsible for this effect is unclear. Objective To identify the effects of aronia consumption on intestinal inflammation and T cell populations in the mouse adoptive transfer model of colitis. Design Recombinase activating gene (RAG) −/− mice were obtained at 4 wk age and were acclimatized for 2 weeks prior to experiments. Live CD4 + CD45RB high cells were obtained by magnetic activated cell sorting (MACS) from gender and age‐matched syngeneic C57BL6 mice and transferred to RAG1 −/− mice by intraperitoneal (IP) injection. Injected RAG1 −/− mice were randomly assigned to receive a control or 4.5% w/w lyophilized ‘Viking’ aronia berry supplemented diet (n = 21/group). A negative control group of RAG −/− mice were injected with sterile phosphate‐buffered saline (PBS) as the sham group (n = 21). Body weight (BW) and food intake were recorded twice a week. Groups of mice were sacrificed at 7–8 wk post injection. Colons were collected for weight and length measurement. Lamina propria lymphocytes (LPL) and mesenteric lymph nodes (MLN) were isolated from colon and analyzed by flow cytometry to determine Th17 and Treg populations. Results BW declined at 4 weeks after CD4 + CD45RB high transfer, and was accompanied by reduced food intakes. The aronia diet prevented colitic weight loss. Control BW decreased to 82.5% at wk 7, whereas the aronia‐fed mice decreased to 93.2% BW at wk 7. Intestinal inflammation was evident by noticeable increases in colon weight/length ratios compared to sham. Aronia consumption inhibited increases in colon weight/length ratios relative to the control (0.042 ± 0.008 vs. 0.058 ± 0.006 g/cm). MLN and LPL Treg populations in both groups of colitic mice did not differ from that in the sham. The total Th17 in LPL but not MLN was higher in aronia group than the control ( P = 0.0216, n = 5–7/group). Conclusion Whole aronia berry consumption reduced wasting in T‐cell transfer‐induced colitis, and increased Th17 but not Treg populations in LPL. Support or Funding Information USDA NIFA AFRI WIS01909