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Potential Effects of javamide‐I found in coffee on inflammatory chemokine MCP‐1 in rats fed a high fat diet.
Author(s) -
Park Jae
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.793.2
Subject(s) - ccl2 , chemokine , p38 mitogen activated protein kinases , monocyte , endocrinology , medicine , kinase , protein kinase a , chemistry , in vitro , pharmacology , inflammation , biochemistry
Javamide‐I is a phenolic amide found in coffee with potential health effects. Monocyte chemoattractant protein‐1 (MCP‐1) is a well‐known chemokine and its reduction is considered beneficial in suppressing the pathophysiological progression of cardiovascular, kidney and other diseases, commonly found in the obese. Therefore, in this paper, we investigated javamide‐I as a potential p38 MAP kinase inhibitor, because the kinase is critically involved in the expression of MCP‐1. At the concentration of 20 μM, javamide‐I significantly inhibited p38 MAP kinase alpha, beta, gamma and delta by 15–50% (P < 0.05), particularly p38 MAP kinase alpha (IC 50 = 19.2 μM) and beta (IC 50 =19.5 μM). We also found that monocytic THP‐1 cells treated with javamide‐I (10, 20 and 40 μM) led to significant inhibition of MCP‐1 production by 17–49% (P < 0.05) in the cells, suggesting that javamide‐I may decrease MCP‐1 expression via inhibiting p38 MAP kinase. Due to the positive in vitro data, the potential effect of javamide‐I on MCP‐1 expression was also investigated in an obesity‐induced rat model. For this study, animals were divided into three groups (n=10): a normal control diet (C), a high fat diet (HF), or a high fat diet supplemented with javamide‐I (2 mg per day) (HFS). After 16 weeks, blood samples were collected from the animals in each group and the plasma levels of MCP‐1 protein were determined. As expected, the average MCP‐1 level of the HFS group was significantly lower than that of the HF group (P < 0.05), although the average MCP‐1 level of the HF group was found to be higher than that of the C group (P < 0.05). In summary, the data suggest that javamide‐I found in coffee may inhibit MCP‐1 expression, possibly via inhibiting p38 MAP kinase and its supplementation could suppress plasma MCP‐1 expression critically involved in several inflammatory diseases. Support or Funding Information USDA (1235‐51000‐054‐00D)