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Activation of Adrenergic Receptor Subtypes Differentially Regulate Expression of Metabolic Genes in Porcine Adipocytes
Author(s) -
Shin Sunhye,
Ajuwon Kolapo M
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.792.16
Subject(s) - endocrinology , medicine , agonist , receptor , adipocyte , adrenergic receptor , peroxisome proliferator activated receptor gamma , adrenergic , biology , coactivator , chemistry , adipose tissue , peroxisome proliferator activated receptor , gene , transcription factor , biochemistry
Pigs adipocytes express mostly the β1 and β2 adrenergic receptors (AR), with limited expression of the β3 adrenergic receptor subtype. However, there is limited understanding of the relative contribution of these receptors to the regulation of important metabolic genes in pig adipocytes. Therefore, this study was conducted with the objective of determining the effect of agonists of β1(dobutamine), β2 (bambuterol), β3 (CGP‐12177‐Hydrochloride) AR and that of α AR (UK14,304). Norepinephrine (NOR) treatment was used to represent non‐specific adrenergic receptor activation. Markers of metabolic activation used include carnitine palmitoyl transferase 1 (CPT1), PPARg coactivator 1a (PGC1a) and deiodinase 2 (DIO2). Significant induction (at least two fold) of expression of these genes was observed with NOR and the b1 agonist, whereas the β2 agonist inconsistently induced these genes. However, the β3 and α AR agonists were ineffective. We further investigated effect of adrenergic receptor blockage with propranolol and inhibition of PKA with H‐89. Both inhibitors blocked NOR and dobutamine effect. These results indicate that pig adipocytes have an inducible, but selective, response to β AR agonists, but are not responsive to α AR agonists. This might indicate the relative importance of these AR subtypes in the regulation of adipocyte metabolism and phenotype in the pig. Support or Funding Information Funding was received from the Department of Animal Sciences, Purdue University