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Effects of collagen peptide on bone in mice with methionine restriction diet
Author(s) -
Guo Haitao,
Wang Yanan,
Zhang Jiahong,
Shi Yonghui,
Le Guowei
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.792.13
Subject(s) - femur , osteocalcin , endocrinology , medicine , methionine , chemistry , surgery , alkaline phosphatase , biochemistry , amino acid , enzyme
High‐fat diets produce many pernicious metabolic consequences including the adverse effects on bone. Methionine restriction (MR) and collagen peptide have already been proved to influence the overall growth of mice. Several studies reported that MR can alters bone morphology including reduced bone mass and collagen peptide can improve bone development. This study was to evaluate the rescue effects of collagen peptide on MR mice bone in male mice. We researched the effects of feeding a 20% fat diet (control), a 20% fat and Methionine restriction diet (MR), a combined MR with 1% collagen peptide (MR/P) for 12 weeks and 23 weeks on bone development in male C57BL/6 mice. MR elevated bone total antioxidant capacity (T‐AOC) and GSH/GSSG, reduced MDA when compared with controls, while MR/P group was not different from MR group. At week 12, the femur length, femur diameter and BMC of MR mice significantly lower than control. However at week 23, the difference could not be observed between MR and control group. But the femur length, femur diameter and BMC of MR/P group were lower than MR group at week 12 and week 23. At week 23, the Distal femur cancelous BMD, BV/TV, Tb.N, Tb.Th was lower in MR/P group and Tb.sp was higher compared with MR group. MR/P group also exhibited decreased expression levels of Runx2, Col1, Bglap2 and reduced bone osteocalcin compared with MR group. These results indicated that MR diet slow down the bone growth rate, but with age the bone development is better than control group and MR/P diet did not improved bone development, but even worsen cancellous bone with MR diet. Support or Funding Information This research was supported by program of “Collaborative innovation center of food safety and quality control in Jiangsu Province”, the National Natural Science Foundation of China (No. 31571841), State Key Laboratory of Food Science and Technology of Jiangnan University in China (SKLF‐ZZB‐201609).