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The proliferation and invasion of low passage number human colon cancer cell lines is inhibited by retinoids
Author(s) -
Martinez Marissa M.,
Hurst Alexandra,
Reynolds C. Patrick,
Lane Michelle A.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.790.4
Subject(s) - retinoid , cell culture , retinoic acid , colorectal cancer , matrigel , cell growth , cancer research , in vivo , metastasis , cell , retinol , cancer cell , receptor , cancer , endocrinology , medicine , biology , chemistry , vitamin , biochemistry , microbiology and biotechnology , genetics
Previously we examined how vitamin A and its metabolite, all‐trans‐ retinoid acid (ATRA), inhibit the proliferation and invasion of commercially available human colon cancer cell lines. Commercial cell lines may be suboptimal models, because they have been in culture for many passages. Our goal was to determine if low‐passage patient‐derived colorectal cancer cell lines (TX‐CC‐208 and TX‐CC‐286) also responded to retinoids. To determine if retinol and ATRA inhibited proliferation, 24 hours after seeding, cells were treated with 0 (vehicle control), 1, or 10 μM retinol or ATRA for 72 hours. TX‐CC‐208 cell number was decreased to 62.1 ± 3.1% of control regardless of retinoid type or concentration. Similarly, the number of TX‐CC‐286 cells was decreased to 51.8 ± 3.9 percent of control. TX‐CC‐208 cells were not invasive. The invasion of TX‐CC‐286 cells through Matrigel‐coated Boyden chambers was decreased to 40 ± 1.2% of control following 72 h of treatment with retinoids regardless of type or concentration. In conclusion, low‐passage (pass 7–17) patient‐derived colorectal cancer cell lines exhibited decreased proliferation and, in one cell line, invasion, in response to retinoid treatment. Both cells lines were sensitive to ATRA, suggesting they express retinoic acid receptors. These receptors are often not found in commercially available colon cancer cell lines, particularly invasive cell lines. These data suggest that retinol and ATRA may decrease the growth and metastasis of colon cancer in vivo . We are currently working to determine if ATRA mediates the ability of retinol to inhibit cell proliferation and invasion. Support or Funding Information This research was supported by donations from the Heather Custer Memorial Fund and Kiel Colon Cancer.