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Charecterization of a Glycerophosphocholine Acyltransferase (Gpc1), a Novel Enzyme in Phosphatidylcholine Biosynthesis in Saccharomyces cerevisiae
Author(s) -
Anaokar Sanket,
Lager Ida,
Glab Bartosz,
Banas Antoni,
Stymne Sten,
PattonVogt Jana
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.781.18
Subject(s) - acyltransferase , biosynthesis , biochemistry , enzyme , acyltransferases , phosphatidylcholine , saccharomyces cerevisiae , biology , chemistry , phospholipid , yeast , metabolic pathway , membrane
Phosphatidylcholine (PC) is the major phospholipid found in most eukaryotic membranes. The maintenance of membrane composition through regulated synthesis and turnover of phosphotidylcholine is crucial to cell functioning. We have identified a novel enzyme glycerophosphosphocholine acyltransferase (Gpc1) that expands our understanding of PC biosynthesis and remodeling. Gpc1 acylates glycerophosphocholine (GPC), a product of complete deacylation of PC to Lysophosphotidylcholine (LysoPC), which can subsequently be converted to PC by lysophospholipd acyltransferase, Ale1. The yeast knock‐out collection was screened in order to find the Gpc1‐encoding gene. In vitro assays confirm that Gpc1 is an acyltransferase involved in PC biosynthesis. In addition, metabolic labeling studies performed in various strain backgrounds in which the established pathways for PC biosynthesis were attenuated indicate an in vivo role for Gpc1 in PC biosynthesis. Factors and growth conditions that regulate GPC1 expression are being explained. Gpc1 belongs to a new protein family as it shows no similarities to the known acyltransferases or transacylases. Gpc1 homologs are found in multiple organism including plants and medically important pathogenic fungi, but they are absent in vertebrates. Overall, we have successfully identified Gpc1, a glycerophosphocholine acyltransferase encoding gene that contributes to PC biosynthesis in the cell. Support or Funding Information National institute of Health Grant NIH R15 GM104876