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Mitochondria‐targeted catalase primes adipocyte differentiation through regulation of Pref‐1
Author(s) -
Tavares Kendall,
Christian Brooke
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.780.13
Subject(s) - catalase , adipocyte , reactive oxygen species , downregulation and upregulation , mitochondrion , microbiology and biotechnology , chemistry , cellular differentiation , peroxisome , lipid droplet , biology , medicine , endocrinology , antioxidant , biochemistry , adipose tissue , gene
Obesity is caused by either an increase in the number of fat cells, an increase in the size of fat cells, or both. Fat cells increase in number when adipocyte precursor cells differentiate into mature fat cells, a process previously acknowledged as dependent on the presence of reactive oxygen species. Antioxidants decrease the amount of reactive oxygen species and reduce adipocyte differentiation. Despite this, a preliminary study found that mitochondria‐targeted expression of the antioxidant catalase caused adipocyte precursors to initially differentiate more quickly than corresponding cells from wild type mice. This increase in differentiation occurred in the first 5 days but was reversed by day 7. Preliminary qRT‐PCR data indicates that cells with mitochondria‐targeted catalase differentiate faster due to the absence of the transcriptional downregulator Pref‐1. The downregulation of Pref‐1 stimulates expression of genes that promote differentiation such as the master regulator PPARγ2 and targets Perilipin, aP2, FASN and AdipoQ. The relationship between catalase and Pref‐1 is currently under investigation. Results from this study may have implications on the potential use of antioxidants as treatments for obesity.