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Increased N‐acetyltaurine in the skeletal muscle after endurance exercise
Author(s) -
Miyazaki Teruo,
Nakamura Yuho,
Ebina Kei,
Ra SongGyu,
Ishikura Keisuke,
Ohmori Hajime,
Ikegami Tadashi,
Matsuzaki Yasushi,
Honda Akira
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.778.4
Subject(s) - taurine , medicine , endocrinology , urine , skeletal muscle , excretion , chemistry , metabolite , soleus muscle , biochemistry , amino acid
Aim Acetate produced from acetyl‐CoA in the liver is utilized for energy production in the skeletal muscle after reconversion to acetyl‐CoA during endurance exercise. However, excess acetate in the muscle might become a nuisance that disturbs normalizing of energy metabolism after the exercise. Therefore, it is suggested that acetate may be metabolized to a novel metabolite N‐acetyltaurine (NAT) by reacting with taurine abundantly contained in the muscle for facilitation of urinary acetate excretion. This study aimed to evaluate NAT in serum, urine, and muscle in human and rats after endurance exercise, and in the cultured muscle cell. Method NAT and taurine levels in serum and urine from healthy volunteers after a transient endurance running and in the soleus muscle from rats after a treadmill running to exhaustion were measured by HPLC‐MS/MS system. The muscular ability of NAT synthesis was also evaluated in C2C12 myotube treated with acetate and taurine. Result Serum taurine and NAT levels were significantly increased immediately after the exercise, and recovered to the basic levels after one day. Urinary excretions of taurine and NAT were markedly higher in the post 24‐hour period. In the rat muscle, NAT concentration was significantly increased correlated with its level in serum after the exercise. When taurine was co‐treated with acetate to the myotube, the significant inhibition of intracellular acetyl‐CoA accumulation and the significant facilitation of extracellular NAT excretion were observed. Conclusion These findings suggest that NAT might be synthesized to excrete the excess acetate from the skeletal muscle into the urine after endurance exercise in order to prevent intramuscular excess accumulation of acetyl‐CoA that might induce a metabolic imbalance after the exercise. Support or Funding Information This study was supported in part by Kakenhi grants (15K09026, 15K08319, 16K01734) from the Japan Society for the Promotion of Science.