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Leigh Syndrome French Canadian Type Patient Fibroblasts Exhibit Energy Metabolism Adaptations Through a Warburg‐like Effect
Author(s) -
Mukaneza Yvette,
Cohen Aaron,
Rivard MarieEve,
Tardif Jessica,
Laprise Catherine,
Des Rosiers Christine,
Coderre Lise
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.778.3
Subject(s) - glycolysis , mtorc1 , oxidative phosphorylation , mitochondrion , warburg effect , protein kinase b , citric acid cycle , pyruvate dehydrogenase complex , biology , pyruvate decarboxylation , pi3k/akt/mtor pathway , cytochrome c oxidase , microbiology and biotechnology , chemistry , phosphorylation , biochemistry , metabolism , signal transduction , enzyme
Background & Objective LSFC is a recessive mitochondrial disease caused by mutations in the LRPPRC gene resulting in a decrease in the LRPPRC protein. This leads to a tissue‐specific reduction of complex IV or cytochrome c oxidase (COX). Our previous work in LSFC fibroblasts demonstrated several mitochondrial functional abnormalities including reduced mitochondrial membrane potential, fragmentation of the mitochondrial network and impaired oxidative phosphorylation capacity. Despite these abnormalities, LSFC fibroblasts did not display any reduction in ATP levels, suggesting the activation of compensatory mechanisms. We hypothesized that mTORC1‐mediated glycolytic ATP production, also known as Warburg effect, might compensate for mitochondrial dysfunction. To address this question, we evaluated glycolytic flux and the Akt/mTORC1 signaling pathway, using LSFC and control skin fibroblasts as a model. Results Our results showed numerous adaptations of LSFC energy metabolism. Metabolic flux analysis revealed that glucose contribution to pyruvate, lactate and alanine formation was respectively increased by 41%, 61%, and 52% (p≤0.05) in LSFC fibroblasts compared to controls. At the molecular level, the Akt/mTORC1 pathway was up regulated in LSFC cells compared to controls (pAkt 111%, p≤0.05; Akt 44%, p≤0.001; mTOR 51%, p≤0.01). This includes enhanced phosphorylation of mTORC1 downstream targets p70S6K by 105% (p≤0.05); as well as an increase in the expression of the transcription factor HIF‐1α by 200% (p≤0.01). The level of pyruvate dehydrogenase kinase 1 (PDHK1), a HIF‐1α downstream target and an inhibitor of mitochondrial glucose oxidation via pyruvate dehydrogenase 1 (PDH1) phosphorylation, was also up regulated by 150% (p≤0.05) in LSFC cells. Consistent with these results, we observed an increase of PDH1 phosphorylation by 94% (p≤0.01) in LSFC cells compared to controls. To further delineate the role of mTORC1 pathway in these adaptations, we inhibited its activity with rapamycin. mTORC1 inhibition did not affect HIF‐1α or PDHK1 expression. However, rapamycin was associated with increased PDH1 phosphorylation (p≤0.05) by 37% and 42% in LSFC and control fibroblasts, respectively. Rapamycin also reduced ATP levels by 8% (p=0.07) in LSFC fibroblasts comparatively to 15% (p≤0.05) in controls. Interestingly, rapamycin decreased LRPPRC expression by 41% (p≤0.01) in LSFC cells compared to only 11% (p≤0.05) reduction in control. This was associated with a selective reduction of COX expression in LSFC (32%, p≤0.01) fibroblasts, while we did not observe any change in expression of the others mitochondrial complexes. Conclusion LSFC fibroblasts maintain ATP levels probably through a “Warburg‐like effect”; for which mTORC1 is not the primary regulator. mTORC1 activation, however, appears important for LRPPRC and therefore, COX expression in LSFC fibroblasts. Support or Funding Information Supported by: Association de l'acidose lactique and CIHR Emerging Team Grant