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Stay On Target: Deconvoluting Mixed Redox Messages through Precision Redox Targeting
Author(s) -
Poganik Jesse R.,
Parvez Saba,
Aye Yimon
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.774.4
Subject(s) - redox , function (biology) , phenotype , computational biology , chemical biology , caenorhabditis elegans , redundancy (engineering) , biology , computer science , chemistry , nanotechnology , microbiology and biotechnology , bioinformatics , biophysics , biochemistry , materials science , gene , organic chemistry , operating system
The precisely timed and spatially regulated chemical signals are the essence of biochemical redox signaling. However, defining the precise biological impacts of localized signals that engage with specific protein targets under physiologic conditions has proven to be highly challenging. This abstract presents a unique set of proximity‐directed chemical tools that enables powerful interrogation into functional consequences of specific redox events through precision redox targeting in living systems (see, for example, Parvez et al. Nature Protocols 2016 11 2328; Long et al. 2016 Nature Chemical Biology Accepted). With this in vivo‐validated redox‐targeting toolset, we identify bona fide “ first responders ” sufficient to drive phenotypic responses at organismal level exemplified by zebrafish and C. elegans models. Our unique chemical biology toolset sets the stage for ruling in gain‐of‐function (or dominant loss‐of‐function) redox modifications and relating them to phenotype in an unbiased experiment that is not hampered by functional redundancy. Support or Funding Information The NSF CAREER, NIH Director's New Innovator, Beckman Young Investigator, and Sloan Fellowship programs are acknowledged for research support.