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Antioxidant and Anti‐proliferative Activity of Fractions from Anona senegalensis Pers (Annonaceae) Stem Bark on HeLa Cells
Author(s) -
Adisa Rahmat Adetutu,
Getti Giulia T.M,
Richardson Simon C.W
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.774.18
Subject(s) - hela , chemistry , antioxidant , dpph , dna fragmentation , mtt assay , apoptosis , fragmentation (computing) , phytochemical , viability assay , biochemistry , traditional medicine , biology , in vitro , programmed cell death , medicine , ecology
Anona Senegalensis Pers (Annonaceae) parts are used locally for herbal therapy, food and aesthetics. There is no scientific record of its anticancer activity on HeLa cells. The aim of the present study was to investigate the antioxidant, cytotoxic and apoptotic properties of the ethanol extracts of Anona senegalensis and its fractions on HeLa cancer cells. The HeLa cells were incubated for 24, 48 and 72 h with different concentrations of fractions of Anona senegalensis for cell viability using the MTT assay. Apoptotic cells were determined by DNA fragmentation and PE‐Annexin V assay using flow cytometry. Phytochemical screening of the ethanol extracts revealed the presence of tannin, saponin, phenol, flavonoid, alkaloid, terpenoids, reducing sugar and cardiac glycosides. Increasing concentrations of the fractions inhibited lipid peroxidation and DPPH radical scavenging activity in a dose‐dependent manner by at least 65% at 100 μg/mL. Furthermore, ethanol, hexane, aqueous, ethylacetate and chloroform fractions inhibited HeLa cells viability to different extent in a concentration‐dependent manner over the incubation periods. The IC 50 values of ethanol, chloroform, hexane, ethylacetate, and aqueous fractions were 235.2, 24.3, 245, 1625.3, and 156.2μg/mL, respectively after 24h. These extract fractions caused apoptosis as shown by DNA fragmentation. Treatment of HeLa cells with the chloroform fraction induced the release of cytochrome C. The results indicate that Anona senegalensis possess strong antioxidant property and anti‐proliferative activity. The chloroform fraction is the most cytotoxic and the mechanism of anti‐proliferative activity involves apoptosis via the mitochondrial pathway through the release of cytochrome c from the mitochondria. Support or Funding Information Federal Republic of Nigeria Tertiary Education Fund (TETFUND) for Academic Staff Training & Development ‐ 2014