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Integrated metabolomic studies on health effects of oxidized tyrosine administration in female mice
Author(s) -
Yang Yuhui,
Yan Biao,
Zhang Tianyu,
Gao Ying,
Shi Yonghui,
Le Guowei
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.769.2
Subject(s) - medicine , endocrinology , choline , creatinine , gut flora , oxidative stress , metabolism , bioavailability , urine , metabolomics , chemistry , biology , biochemistry , pharmacology , bioinformatics
Oxidized tyrosine (O‐Tyr) which is associated with some pathological conditions and metabolic disorders (especially various age‐related disorders) in biological system, can be easily detected in protein‐rich food systems. However, the systemic effects of O‐Tyr in the animal body is unexplored. This study aims to investigate the effects of O‐Tyr on the overall metabolic status of mice. Twenty female Kunming mice were intragastrically administered with saline or O‐Tyr (320 μg/kg body weight) every day for six weeks. Urine and serum were sampled and analyzed by 1 H nuclear magnetic resonance‐based metabolomics and coupled with multivariate data analysis methods. Body weight, serum biochemical profiles, organs index and histopathological data were obtained as complementary information. Compared with control group, O‐Tyr administration had a tendency to increase the body weight of mice (P>0.05), enhanced the activities of serum alanine aminotransferase and aspartate aminotransferase and the level of serum creatinine, and decreased the level of serum total protein (P<0.05). O‐Tyr administration changed systemic metabolic processes, including inhibited energy metabolism and the biosynthesis of protein, increased oxidative stress, elevated metabolism of vitamin‐B 3 , altered gut microbiota functions, decreased choline bioavailability. In addition, O‐Tyr administration also impaired liver and kidney function, lead to fat accumulation in liver. We concluded that metabolisms of vitamin‐B 3 and choline together with gut microbiota may played crucially important roles in the deleterious effects of O‐Tyr. These findings provided a comprehensive and holistic perspective on the safety of O‐Tyr, and revealed the need for reduce the generation of oxidation protein products in food production process by improving the processing methods. Support or Funding Information This research was supported by program of “Collaborative innovation center of food safety and quality control in Jiangsu Province”, the National Natural Science Foundation of China (No. 31571841), State Key Laboratory of Food Science and Technology of Jiangnan University in China (SKLF‐ZZB‐201609), and China Postdoctoral Science Foundation (2015M571669).