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ANNONA SENEGALENSIS SHOWS POTENT ANTI‐DIABETIC ACTIVITY BY ATTENUATING DNA FRAGMENTATION IN OXIDATIVE INJURY AND INHIBITING KEY‐ENZYMES LINKED TO TYPE 2 DIABETES
Author(s) -
Erukainure Ochuko L.,
Islam Shahidul
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.766.7
Subject(s) - chemistry , antioxidant , dpph , superoxide dismutase , catalase , traditional medicine , glutathione , oxidative stress , malondialdehyde , biochemistry , pharmacology , enzyme , biology , medicine
The leaves and stem bark of Annona senegalensis were investigated for their anti‐oxidative vis‐à‐vis anti‐diabetic potentials in vitro. Extracts from sequential extraction with solvents of increasing polarity (n‐hexane, ethyl acetate, ethanol and aqueous) of the leaves and stem barks were subjected to in vitro antioxidant assays using the 2,2′‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging and Ferric reducing antioxidant power (FRAP) protocols respectively. Their inhibitory effects were investigated on intestinal α‐glucosidase, hepatic and pancreatic lipases, glucose‐6‐phospatase and pancreatic ATPase activities. Their antioxidant and anti‐apoptotic effects on Fe 2+ ‐ induced oxidative injuries in pancreatic and hepatic tissues were also investigated ex vivo. The extracts showed potent free radical scavenging activity and significantly (p<0.05) inhibited all studied enzymes, with the stem bark extract showing better activity. Superoxide Dismutase (SOD) and Catalase activities were significantly (p<0.05) increased in both pancreatic and hepatic tissues with concomitant elevation of reduced glutathione (GSH) levels as well as reduced levels of malondialdehyde (MDA). The extracts significantly inhibited DNA fragmentation in both tissues. These activities were dose – dependent. These results indicate the anti‐oxidative, anti‐diabetic and anti‐obesogenic potentials of A. senegalensis , with the ethanolic extract of stem bark showing the best activity. Thus, giving credence to its antidiabetic folkloric claims Support or Funding Information University of KwaZulu‐Natal, Durban 4000, South Africa