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Electrochemical detection of isatin using flow injection analysis with amperometric detection
Author(s) -
Sanchez Simon Wolfgang,
Jarosova Romana,
Swain Greg M
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.766.16
Subject(s) - isatin , amperometry , electrode , detection limit , analytical chemistry (journal) , chemistry , glassy carbon , cyclic voltammetry , electrochemistry , materials science , chromatography , organic chemistry
Isatin is a heterocyclic compound that has been shown to have a wide range of biological activities. Isatin is found endogenously in humans and rodents. In rat models, isatin concentrations have been shown to increase with stress in the heart, brain, blood plasma, and urinary samples. In the rat, highest concentrations are in the vas deferens and seminal vesicles with levels in the heart somewhat higher than in the brain. The highest concentrations in the brain are found in the hippocampus and cerebellum. Isatin is electrochemically active and can be detected with electrochemical techniques. Of these techniques, flow injection analysis is a versatile technique used for the determination of easily oxidizable or reducible analytes. The performance of a nitrogen‐incorporated tetrahedral amorphous carbon electrode and a boron‐doped diamond electrode was evaluated using flow injection analysis with amperometric detection. The boron‐doped diamond electrode is known for its excellent properties such as a low stable background current, weak molecular absorption, and microstructural stability, but has a high deposition temperature required for growth, (600–800 °C). Similarly, the ta‐C:N electrode has been shown to exhibit many of the same attractive properties of the boron‐doped diamond electrode such as a low background current, microstructural stability, and weak molecular absorption with the advantage of a low deposition temperature near room temperature (25–100 °C). The analytical detection figures of merit such as the response precision, sensitivity, linear dynamic range and limit of detection for isatin in a 0.1 M phosphate buffer (pH 7.2) were determined for both electrodes.