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Identification of Tumor Necrosis Factor‐α Inhibitors from the Traditionally Used Chinese Plant Mappianthus iodoides
Author(s) -
Garrison Meagan,
Park Hyo,
Wright Matt,
Farone Anthony
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.766.1
Subject(s) - cytotoxicity , tumor necrosis factor alpha , pharmacology , viability assay , anti inflammatory , traditional medicine , chemistry , medicinal plants , necrosis , biology , cell , biochemistry , medicine , in vitro , immunology , genetics
The inflammatory response is designed to protect the human body from foreign pathogens, including bacteria, fungi, virus, and parasites. However, when inflammation is uncontrolled it can lead to serious inflammatory and autoimmune diseases. The tumor necrosis factor‐α (TNF‐α) inhibiting drugs currently used as treatment for these diseases, primarily non‐steroidal anti‐inflammatory drugs (NSAIDs), have severe side effects; therefore, there is a desperate need for less toxic, alternative treatments. Traditional Chinese Medicine has grown in popularity in the United States over recent years and many plants are being studied for their medicinal purposes. In the present study, 12 fractions of one Chinese plant, Mappianthus iodoides , were screened for cytotoxicity to human macrophage‐like cells using an Alamar Blue cell viability assay and the non‐toxic concentrations were then assayed for TNF‐α inhibitory activity using enzyme‐linked immunosorbent assay (ELISA). Two fractions exhibited >90% inhibition of TNF‐α at 50 μg/mL, four fractions showed inhibition at 25 μg/mL, and two fractions inhibit at 10 μg/mL and 5 μg/mL. Fraction 7 exhibited greater than 90% inhibition of TNF‐α at 5 μg/mL and is being further fractionated into five isolated compounds using High Performance Liquid Chromatography (HPLC). The five isolated compounds will be characterized and identified using Mass Spectrometry and then assayed for cytotoxicity and TNF‐α inhibition to determine whether the pure compounds inhibit inflammation and may therefore be a potential alternative drug. Support or Funding Information Tennessee Center for Botanical Medicine Research (TCBMR) Guangxi Botanical Garden of Medicinal Plants (GBGMP)