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Effects of Dietary Isoflavones, Age, and Stroke on Estrogen Pathway Activation in Male Rats
Author(s) -
Hart Muriel Lynn,
Huber Kalene,
Grisley Elizabeth Dawn,
Cheatwood Joseph L
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.747.3
Subject(s) - genistein , daidzein , neuroprotection , isoflavones , medicine , stroke (engine) , estrogen receptor , endocrinology , phytoestrogens , soy isoflavones , estrogen , pharmacology , cancer , mechanical engineering , breast cancer , engineering
The effects of stroke can cause long lasting impairment on not only one's physical health but their quality of life and everyday function. Dietary modifications provide a potential solution to aid in neuroprotection and allow enhanced functional recovery post‐stroke. Previous investigation tested this, and found the addition of dietary soy compounds does show significantly improved functional recovery through behavioral tests in our rat models. Further studies aimed to identify specific effects on gene expression on the dietary inclusion of isoflavones alone, using Daidzein and Genistein, known neuroprotective estrogenic receptor activators. We not only examined the effect of isoflavone content on altered gene expression, but also age as another potential mediating factor either promoting or inhibiting anti‐inflammatory and neuroprotective pathways. In doing so, both adult and aged male Hooded Long Evan rats were separated into distinct dietary protein groups containing either Sodium Caseinate (CAS), or Sodium Caseinate with the isoflavones daidzein and genistein (CAS+ISO) two weeks before a permanent unilateral middle cerebral artery occlusion (MCAO). Tissue samples were collected from the injured hemisphere on Day +3 (n=6) and Day +0 (n=4; received no MCAO). Data were collected via a qPCR array after RNA extraction and cDNA synthesis, following the manufacturer's protocol guidelines (Qiagen). Expression of mRNA was compared between treatment groups based on age, isoflavone content, and stroke. Changes in mRNA expression greater than 2‐fold were considered significant. Detailed results will be presented on the poster. Support or Funding Information NIH NCCAM (1R15AT006593; JLC) and DuPont Nutrition and Health