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The Pattern of VEGF Expression in Avian Renal Development
Author(s) -
McNeil Rosie Thecla,
Penny Clement Brian
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.740.6
Subject(s) - vascular endothelial growth factor , kidney development , biology , angiogenesis , kidney , pathology , microbiology and biotechnology , immunohistochemistry , basement membrane , immunology , vegf receptors , endocrinology , medicine , gene , cancer research , genetics , embryonic stem cell
Vascular endothelial growth factor (VEGF) is a major regulator of mammalian blood vessel biology and its expression has been implicated in both pathological and developmental angiogenesis. Its role in glomerular biology has remained elusive since the general concepts of mesenchymal‐epithelial interactions even in mammalian kidney development is not well known. This lack of knowledge maybe because early patterning of the kidney region depends on interactions between several genes. Studies in chick embryos and of genes identified in human renal diseases have contributed to knowledge of early patterning of the kidney region and the early events that lead to the development of the unique glomerular basement membrane of the Kidney. The aim of this work is to ascertain the pattern of Vascular endothelial growth factor (VEGF) expression in avian renal development. Tissue sections of 5 μm thickness were obtained from paraffin‐embedded thoracic spinal segments at developmental stages 19, 22, 25, 27, 29, 33, 37 and 40 (Hamburger and Hamilton staging for avian development). They were then tested for cytoplasmic staining by standard Immunohistochemistry, using VEGF A antibodies (primary goat anti‐rabbit antibodies), detected with secondary Alexa Fluor® 568, red (Goat Anti‐Rabbit IgG, 1: 300 dilution SC) and viewed on an Olympus IX71 inverted fluorescent microscope. Images were captured using Olympus analysis Software. The results showed that VEGF expression is required from as early as stage 22, increasing with intensity with developmental maturity. This finding that implies that vascular endothelial growth factor plays a significant role in early renal development and function. Support or Funding Information We wish to thank the University of the Witwatersrand Johannesburg for the postgraduate merit award and also, the Faculty of Health Sciences, for the research grant which made this work possibleStage 22, the developing kidneys with marked VEGF expression