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Bioactive Fibrin Scaffolds for Use in Regenerative Medicine
Author(s) -
Braga Sarah Felicio,
Aquino Renata,
Carvalho Antonio,
Trovatti Eliane,
Iemma Mônica,
Amaral André Capaldo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.731.3
Subject(s) - fibrin , viability assay , regenerative medicine , platelet rich fibrin , chemistry , matrix (chemical analysis) , fibroblast , platelet rich plasma , biomedical engineering , tissue engineering , microbiology and biotechnology , cell , in vitro , immunology , medicine , biology , biochemistry , platelet , chromatography
Regenerative medicine has been shown to be a promising approach for treatment of musculoskeletal injuries, using as biological strategies scaffolds, cell therapy and biomolecules. The autologous fibrin matrix, derived from leukocyte (LEU) and platelet (PLA)‐rich plasma (L‐PRP) and LEU and PLA‐rich fibrin (L‐PRF) techniques, presents a great potential to act as a bioactive support in regenerative medicine, being able to contribute with the intrinsic maintenance of the cellular viability, stimulation of proliferation and the repopulation potential in the injury site. The aim of this research was to characterize the intrinsic cell viability maintenance potential of different bioactive fibrin scaffolds containing PLA and LEU. For such, blood samples from 3 male volunteers were collected and processed to obtain fibrin clots from the L‐PRP and L‐PRF techniques, using a fibrin matrix devoid of PLA and LEU as a control. In order, to characterize the in vitro viability potential, the human fibroblast cell line (MG07492) was used. After the sowing procedure (10 4 cells), the scaffolds were maintained for 5 days in standard culture conditions in serum‐free Dulbecco's Modified Eagle Medium and subsequently submitted to analysis by scanning electron and fluorescence microscopy. The results demonstrated a distinct potential to promote intrinsic viability between L‐PRP and L‐PRF scaffolds, with L‐PRP responsible for the highest levels of viability. No cells were identified in the fibrin matrix used as control. These results allow the conclusion that fibrin scaffolds from the L‐PRP and L‐PRF techniques present intrinsic cell viability potential and represent promising alternatives for use as bioactive scaffold in musculoskeletal regenerative medicine. Support or Funding Information CNPq‐ Conselho Nacional de Desenvolvimento Científico e Tecnológico.