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Basal Ventilatory Activity in Conscious Freely‐moving Spontaneously Breathing 6‐OHDA SN‐lesioned Parkinson's Disease Rat Model
Author(s) -
Wadolowski Robert M,
Wadolowski Steven B,
Collins William F,
Solomon Irene C
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.728.14
Subject(s) - parkinson's disease , substantia nigra , basal (medicine) , respiratory system , basal ganglia , medicine , control of respiration , lesion , tidal volume , breathing , endocrinology , cardiology , neuroscience , chemistry , anesthesia , pathology , psychology , central nervous system , disease , insulin
Respiratory dysfunction in Parkinson's Disease (PD) patients manifests as a variety of altered breathing patterns that are suggested to result from impairment of central respiratory control. Ongoing work in our laboratory has recently identified time‐dependent alterations in basal inspiratory motor output and chemical control of breathing in the 6‐hydroxydopamine (6‐OHDA) neurotoxin‐induced unilateral substantia nigra (SN) lesion rat model. Our studies, however, have been limited to examination of these ventilatory behaviors at specified time points in acute terminal experiments; thus, the time course underlying the progression of the changes observed is not known. Here, we begin to address this issue by examining the basal respiratory phenotype using whole body plethysmography before and weekly for 4‐weeks after administration of 6‐OHDA into the SN of adult female Sprague‐Dawley rats; vehicle injected rats served as a control. Our preliminary data from 6‐OHDA SN‐lesioned rats (compared to vehicle control rats) suggest that there is a progressive reduction in tidal volume (TV) with a slight decrease in peak expiratory flow as indicated by a reduced EF50 and relaxation time albeit these changes are fairly modest. In addition, 6‐OHDA SN‐lesioned rats appear to exhibit a progressive reduction in variability in most of the time series respiratory parameters examined (e.g., TV, expiratory volume, EF, PIF, PEF, MV). These observations suggest that in conscious freely‐moving 6‐OHDA SN‐lesioned rats, ventilatory behaviors undergo a progressive alteration; however, the 4‐week time course studied here was only able to identify limited changes. We suggest that additional experiments examine a longer post‐lesion time period in order to more fully characterize the time course underlying the progression of ventilatory impairment in this PD rat model. Support or Funding Information Thomas Hartman Center for Parkinson's Disease Research at Stony Brook University