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Effect of resistance exercise training in mitophagy of diabetic rats
Author(s) -
Jorge luciana,
Silva Kleiton Augusto,
Luiz Rafael S,
Rampaso Rodolfo R,
Schor Nestor
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.716.1
Subject(s) - microalbuminuria , medicine , diabetic nephropathy , diabetes mellitus , glycemic , endocrinology , mitophagy , muscle hypertrophy , pathogenesis , urology , apoptosis , biology , biochemistry , autophagy
Diabetic nephropathy (DN) is a progressive complication arising from diabetes. The repertoire of kidney cell response to injury includes cell division, cell hypertrophy, apoptosis and mitochondrial dysfunction. Increasing evidence indicates that the disruption of mitochondrial may be important in the development and progression of DN. On the other hand, the resistance exercise training is fundamental as non‐pharmacological intervention to prevent those complications. In this study, we investigated the effect of resistance exercise training (RET) on the DN pathogenesis. Male Wistar rats were divided into 4 groups: control (C=8), control trained (CT=8), diabetic (D=8) and diabetic trained (DT=8). Diabetes Mellitus (DM) (glycemia>250mg/dL) was induced by streptozootocina (STZ) (50mg/kg, i.v.). Trained groups were submitted to resistance exercise training protocol on ladder climb for 8 weeks. DN were accompanied by urinary volume and microalbuminuria and the expression of DRP‐1 and LC3 protein was evaluated by western blotting. The results indicate RET improved renal parameters in the DT group showing a decreased urinary volume (DS:160; DT:120; CS:13 and CT:14ml), microalbuminuria (DS:8.7; DT:4.7; CS:1.5; CT:2.3mg/24h). The renal expression of DRP‐1 and LC3 were enhanced in DS group and RET normalized this expression. Our findings demonstrated that RET increases in physical capacity and mitochondrial and renal benefits following training protocol, regardless of any improvement in glycemic control Support or Funding Information CNPQ, CAPES, Fapesp, FOC