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Whole Body Hyperthermia Enhances Angiogenesis in Tenotomized Rat Skeletal Muscle
Author(s) -
Hirunsai Muthita,
Srikuea Ratchakrit
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.710.9
Subject(s) - tenotomy , angiogenesis , soleus muscle , skeletal muscle , vascular endothelial growth factor , hyperthermia , medicine , endocrinology , muscle atrophy , anatomy , tendon , vegf receptors
In response to disuse, skeletal muscle is characterized by a reduction of fiber size, increase of intramuscular fibrosis, and regression of capillaries. Hyperthermia has been known to alleviate muscle atrophy and remodel extracellular matrix. Of these, the mechanisms underlying the beneficial effects of heat treatment on capillary adaptation following disuse atrophy are unknown. In the current study, we examined the effects of hyperthermia on angiogenesis and expression of the angiogenic factors in the soleus muscle following Achilles tendon ablation (tenotomy). Male Wistar rats were randomly assigned into three groups: control (CON), tenotomy for 8 day without heat treatment (TEN), and tenotomy for 8 day plus heat treatment (TEN+HEAT). Whole body hyperthermia was maintained at 40.5–41.5°C for 30 min, 24 h before and after tenotomy, and continued for five consecutive days. Following tenotomy, soleus muscle revealed a reduction in muscle fiber size and a remarkable loss of intramuscular capillary. These occur accompanied with decrease in vascular endothelial growth factor receptor‐2 (VEGFR‐2), the pro‐angiogenic factor and increase in thrombospondin‐1 (TSP‐1), the anti‐angiogenic factor, protein expressions in soleus muscle. However, tenotomy had no effect on the protein expression of vascular endothelial growth factor (VEGF). To rescue the capillary loss, whole body hyperthermia preserved the level of capillarization associated with increased VEGFR‐2 and reduced TSP‐1 protein expressions in tenotomized soleus muscle. Whereas, hyperthermia had no additional effect on VEGF protein level in soleus muscle after tenotomy. Collectively, whole body hyperthermia mitigated the effect of tenotomy‐induced capillary regression, in part, by modulating the angiogenic regulators. The results from this study suggest the potential therapeutic effect of heat treatment in relieving intramuscular capillary regression during skeletal muscle atrophy. Support or Funding Information The research was supported by grant from Srinakharinwirot University.