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Changes in the Oxyhemoglobin Dissociation Curve and Cerebral Oxygenation During Acute Systemic Inflammation in Healthy Volunteers
Author(s) -
Dahl Rasmus,
Plovsing Ronni,
Berg Ronan
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.705.4
Subject(s) - oxygen–haemoglobin dissociation curve , oxygenation , medicine , anesthesia , venous blood , systemic inflammation , hemoglobin , arterial blood , inflammation , oxygen , sepsis , oxygen tension , chemistry , organic chemistry
Background Acute systemic inflammation, as encountered in patients with sepsis in the clinical setting, has previously been found to be associated with a progressive decrease in brain tissue oxygen tension (Taccone et al. 2014). In the present study, we used an established human‐experimental model of acute systemic inflammation to investigate whether this involves a reduction in the oxygen availability to the brain tissue by means of configurational changes in the oxyhemoglobin dissociation curve (ODC). Methods Nineteen healthy male volunteers received a four‐hour intravenous lipopolysaccharide (LPS) infusion (total dose 2 ng kg −1 ). Nine of the volunteers (group 1) were poikilocapnic, while CO 2 was added to the inspired air in ten of the volunteers (group 2). Paired arterial‐jugular venous blood samples were obtained. From these the P 50 and Hill's coefficient of hemoglobin were calculated on the basis of a mathematical model of the ODC (Dash et al. 2016), and adjusted to the cerebral capillary level for the calculation of the oxygen tension P capO2 (Gjedde 2005). Results LPS induced configurational changes in the ODC which became more hyperbolic with a consequent left‐shift at the cerebral capillary level, changes that were notably pronounced in group 1 ( Figure 1). Even though the cerebral oxygen delivery and extraction fraction, arterial‐to‐jugular venous oxygen differences, as well as arterial oxygenation were unaffected after LPS, P capO2 was reduced in both groups ( Figure 2), and more so in group 1 than in group 2 (p < 0.05). Conclusion Configurational changes in the ODC which are facilitated by hyperventilation are likely to contribute to a reduction in cerebral oxygenation during acute systemic inflammation. Support or Funding Information The study was supported by The Foundation of Merchant Jakob Ehrenreich and Grete Ehrenreich, The Foundation of 1870, Christian Larsen and Judge Ella Larsen's grant, The Classen Trust Jubilee Foundation, The P. Carl Petersen Foundation, University Hospital Rigshospitalet, and the Faculty of Health Sciences, University of Copenhagen.