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Adenosine A 1 receptor‐operated calcium entry in renal afferent arteriolar smooth muscle cells is dependent on postnatal maturation of canonical transient receptor potential isoform 3 channels
Author(s) -
PeixotoNeves Dieniffer,
Soni Hitesh,
Buddington Randal K,
Adebiyi Adebowale
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.701.5
Subject(s) - trpc3 , afferent arterioles , medicine , endocrinology , transient receptor potential channel , adenosine , receptor , phospholipase c , kidney , trpc , ccpa , chemistry , protein kinase c , biology , adenosine receptor , microbiology and biotechnology , angiotensin ii , signal transduction , agonist
Adenosine, a regulator of cardiovascular development and renal function constricts renal afferent arterioles by activating its cognate A 1 receptors (AA 1 Rs). Activation of AA 1 Rs elevates intracellular Ca 2+ ([Ca 2+ ] i ) concentration in renal vascular smooth muscle cells, mechanisms of which are not fully resolved. Whether AA 1 R expression and function in preglomerular microvessels are dependent on postnatal kidney maturation are also unclear. Here, we show that selective activation of AA 1 R by 2‐chloro‐N 6 ‐cyclopentyladenosine (CCPA) does not stimulate store‐operated Ca 2+ entry in afferent arterioles isolated from infant pigs. However, CCPA‐induced [Ca 2+ ] i elevation is dependent on phospholipase C, protein kinase C, and canonical transient receptor potential isoform 3 (TRPC3) channels. The basal [Ca 2+ ] i concentration was unchanged in afferent arterioles isolated from newly farrowed (0 day) pigs when compared with their 20‐day old counterparts. Whereas, CCPA‐induced [Ca 2+ ] i elevation was ~ 43% larger in afferent arterioles from 20‐day old pigs. AA 1 R protein expression levels in the kidneys and afferent arterioles were unaltered in 0‐day versus 20‐day old pigs. By contrast, TRPC3 channel protein expression was ~ 92 and 78% higher in 20‐day old pig kidneys and afferent arterioles, respectively. These data suggest that activation of AA 1 Rs elicits receptor‐operated Ca 2+ entry in porcine afferent arterioles, an effect dependent on postnatal maturation of TRPC3 channels. Support or Funding Information NIH R01 DK101668