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Assessment of Protective Effect of Phikud Navakot Extract against Hypoxia/Reoxygenation Injury on H9c2 Cardiomyoblasts
Author(s) -
Gerdprasert Orapin,
Nusuetrong Punnee
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.699.2
Subject(s) - cytotoxicity , hypoxia (environmental) , reactive oxygen species , intracellular , andrology , pharmacology , mtt assay , medicine , chemistry , oxygen , traditional medicine , cell , biochemistry , in vitro , organic chemistry
Phikud Navakot is composed of nine main medicinal plants in Yahom Navakot, which has been used as traditional medicine for treatment of cardiovascular symptoms such as dizziness and fainting. The present study was to investigate the protective effect of the ethanolic extract of Phikud Navakot (PN) against hypoxia/reoxygenation (H/R)‐injured H9c2 cardiomyoblasts. The cytotoxicity of PN (0.001–0.5 mg/mL) on H9c2 cells was measured by MTT assay, showing no cytotoxicity to H9c2 cells. Cardioprotective ability of PN was investigated during exposure to 6‐h hypoxic buffer in ischemic box followed by 24‐h reoxygenation in complete medium in CO 2 incubator. The results showed that PN at a concentration of 0.1 mg/mL was able to increase the percentage of cell survival in H/R‐induced cell death when compared with the H/R control, using 6‐hydroxy‐2,5,7,8‐tetramethylchroman‐2‐carboxylic acid as a positive control. PN at the same concentration was also decreased the intensity of reactive oxygen species (ROS) production measured by 2′,7′‐dichlorofluorescin diacetate, when compared to the H/R control. In conclusion, the present results revealed that PN (0.1 mg/mL) was able to decrease H/R‐induced cell injury in H9c2 cardiomyoblasts, which should be partly through inhibition of the intracellular generation of ROS. Support or Funding Information This work was supported by research grant from the National Research Council of Thailand (NRCT 2009‐113). We specially thank Dr. Sanya Hokputsa for giving us the extracts. The authors gratefully thank Mr. Weerasak Ussawawongaraya for preliminary of hypoxic‐simulated H9c2 cells.