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The Therapeutic Potential of Modified Deep‐Ocean Water on 2,4‐dinitrochlorobenzene Induced Atopic Dermatitis in Balb/c Mice
Author(s) -
Ho ChingJung,
Chien ChiangTing
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.697.9
Subject(s) - atopic dermatitis , spleen , medicine , balb/c , white pulp , inflammation , immunology , immune system
Atopic dermatitis is a chronic skin disease characterized with continuous inflammation and pruritic skin lesions. Repeating epicutaneous application of 2,4‐dinitrochlorobenzene (DNCB) treated on the dorsal skin of Balb/c mice can induce atopic dermatitis‐like symptoms and skin lesions. Deep ocean water (DOW), which is rich in micronutrients and minerals and with antioxidant and anti‐inflammatory qualities, may be developed as marine drugs to provide skin protection against atopic dermatitis. Because of the unique properties of DOW, in the present study, we explored its therapeutic potential on DNCB‐induced atopic dermatitis in Balb/c mice. The DOW with hardness at 2400 ppm was orally administrated for 2 weeks to the mice. Our results showed that there was no significant difference in scratching behavior between DNCB group and DOW group. However, the skin wound caused by DNCB was healing sooner in DOW group compared to tap‐water‐treated group. In histopathological analysis, oral DOW administration significantly alleviated DNCB‐induced epidermal thickness and lymphocyte extravasation. Caspase‐3, one of the apoptosis related proteins, was significantly increased in DNCB‐induced dermatitis skin tissue. The enhanced caspase‐3 expression was efficiently decreased after oral DOW treatment. Spleen, an organ plays important roles in atopic dermatitis. The spleen weight of DNCB group was significantly higher. However, the spleen weight did not decrease in DNCB group with DOW treatment. We also found that the margin between red pulp and white pulp of spleen was blurred in DNCB group but was alleviated in DOW group. In conclusion, these results suggest that oral DOW treatment may be a potentially therapeutic strategy in ameliorating atopic dermatitis.