Premium
The Effects of Conjugated Linoleic Acid and Berberine Supplementation on Markers of Oxidative Stress in Broiler Chickens
Author(s) -
Dartigue Vincent,
Klandorf Hillar,
Falkenstein Elizabeth,
Barnes Kimberly,
Van Dyke Knox
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.696.1
Subject(s) - allopurinol , oxidative stress , chemistry , tbars , uric acid , conjugated linoleic acid , broiler , glutathione , berberine , lipid peroxidation , biochemistry , reactive oxygen species , endocrinology , medicine , linoleic acid , fatty acid , biology , food science , enzyme
Reactive oxidative species (ROS) are oxygen base molecules that are leaked from the electron transport chain during mitochondrial cellular respiration. They are kept in balance by endogenous and exogenous antioxidants, which may include conjugated linoleic acid (CLA) and a plant root extract called berberine. A redox shift towards oxidants causes a state called oxidative stress, which is detrimental if prolonged. A ten week study examined the effect CLA and berberine supplementation on markers of oxidative stress in broiler birds. Broilers (n=60) were divided into six groups; the diet of group four to six included allopurinol (25mg/kg body weight) which induced an oxidative stress state. Pen 1(P1) served as the control, P2 CLA oil (50% substitution), P3 berberine supplementation (20g/kg feed), P4 allopurinol alone, P5 CLA oil + allopurinol and P6 berberine + allopurinol. Body weight, plasma uric acid as a marker of oxidative stress, blood glucose, lipid peroxidation via TBARs assay of serum, and relative gene expression of five endogenous liver antioxidants were measured during the course of the study. The addition of allopurinol to the diet induced an oxidative stress state when compared to the baseline groups as observed by a significant reduction in plasma uric acid. In P5 and P6 treatment we observed on average a 10 fold increase in the relative mRNA expression of superoxide dismutase 2 and 3 as well as glutathione 3 and 7. Notably, CLA increased the expression of uncoupling protein 34 fold compared to control while the addition of allopurinol blocked these changes; in contrast there was a slight increase in the berberine+allopurinol treatment. No significant changes were measured in blood glucose and TBARS. Despite the increase in mRNA expression of the antioxidants genes, the result of the uric acids assay suggest that at the dosages administered CLA and berberine were not effective in reversing the oxidative stress induced by allopurinol. Support or Funding Information WVU Hatch