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P66Shc‐induced MicroRNA‐34a Causes Diabetic Endothelial Dysfunction by Downregulating Sirtuin1
Author(s) -
Gabani Mohanad,
Li Qiuxia,
Kim YoungRae,
Vikram Ajit,
Kumar Santosh,
Kassan Modar,
Kim Sang Ki,
Jacobs Julia,
Irani Kaikobad
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.689.3
Subject(s) - downregulation and upregulation , endothelial dysfunction , endothelium , oxidative stress , gene knockdown , sirtuin 1 , endothelial stem cell , chemistry , endocrinology , medicine , in vitro , biochemistry , apoptosis , gene
Objective Diabetes mellitus causes vascular endothelial dysfunction and alters vascular microRNA expression. We investigated if endothelial microRNA‐34a (miR‐34a) leads to diabetic vascular dysfunction by targeting endothelial Sirtuin1 (Sirt1), and asked if the oxidative stress protein p66Shc governs miR‐34a expression in the diabetic endothelium. Approach and Results MiR‐34a is upregulated, and Sirt1down‐regulated, in aortic endothelium of db/db and streptozotocin (STZ)‐induced diabetic mice. Systemic administration of miR‐34a inhibitor, or endothelium‐specific knockout of miR‐34a, prevents downregulation of aortic Sirt1 and rescues impaired endothelium‐dependent aortic vasorelaxation induced by diabetes. Moreover, overexpression of Sirt1 mitigates impaired endothelium‐dependent vasorelaxation caused by miR‐34a mimic ex vivo . Systemic infusion of miR‐34a inhibitor or genetic ablation of endothelial miR‐34a prevents downregulation of endothelial Sirt1 by high glucose. MiR‐34a is upregulated, Sirt1 is downregulated, and oxidative stress (H 2 O 2 ) is induced in endothelial cells incubated with high glucose, or the free fatty acid palmitate in vitro . Increase of H 2 O 2 and induction of endothelial miR‐34a by high glucose or palmitate in vitro is suppressed by knockdown of p66shc. In addition, overexpression of wild‐type but not redox‐deficient p66Shc upregulates miR‐34a in endothelial cells. P66Shc‐stimulated upregulation of endothelial miR‐34a is suppressed by cell‐permeant antioxidants. Finally, mice with global knockdown of p66Shc are protected from diabetes‐induced upregulation of miR‐34a, and downregulation of Sirt1, in the endothelium. Conclusions These data show that hyperglycemia and elevated free fatty acids in the diabetic milieu recruit p66Shc to upregulate endothelial miR‐34a via an oxidant‐sensitive mechanism, which leads to endothelial dysfunction by targeting Sirt1. Support or Funding Information NIH grant T32 HL007344 NIH grant T32 HL007121 University of Iowa Endowed Professorship in Cardiovascular Medicine