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Contribution of Adiponectin to Vascular Responses in Bone Resistance Arteries in Mice
Author(s) -
Ghosh Payal,
Hotta Kazuki,
Lucero Tiffany,
Borodunovich Kyle,
Cowan Morgan,
Bramy Jeremy,
Behnke Bradley,
Delp Michael,
Delp Judy
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.684.16
Subject(s) - vasodilation , medicine , endocrinology , adiponectin , vasomotor , endothelium , vascular resistance , hemodynamics , insulin resistance , obesity
Adiponectin has been reported to alter structural properties of bone, which may be influenced by altered regulation of bone blood flow and related vasomotor properties in the bone resistance vasculature. Therefore, the purpose of this study was to determine the effects of adiponectin deletion on bone resistance arterial function. Adult male C57BL/6 wild‐type (WT) and homozygous adiponectin knockout (AdipoKO) mice were obtained for study. Femoral principle nutrient arteries (PNA) were isolated, cannulated and pressurized for in vitro study. Endothelium‐dependent (ACh, 10 −9 –10 −4 M) and ‐independent (Dea‐NONOate, 10 −9 –10 −4 M) vasodilation, and myogenic (0 to 130 cmH 2 O) responsiveness were evaluated. Endothelium‐dependent vasodilation was significantly impaired in PNA arteries from AdipoKO mice compared to arteries from WT mice; however, no significant differences in endothelium‐independent vasodilation were observed between arteries from WT and AdipoKO mice. Myogenic responsiveness tended to be greater in arteries from AdipoKO mice than arteries from WT mice. The greater myogenic tone seen in PNA arteries from AdipoKO mice may be linked to endothelial dysfunction. These results indicate that in bone resistance arteries, adiponectin modulates endothelium‐dependent vasodilation but does not affect vasodilatory function of the vascular smooth muscle. These alterations in vasomotor function could potentially play a role in alterations of bone structural properties associated with changes in circulating adiponectin.