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In Vitro Assessment of Drug Interaction Potential of Licorice Extracts
Author(s) -
TonsingCarter Alyssa,
Li Guannan,
Breemen Richard
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.669.16
Subject(s) - cyp3a4 , cyp1a2 , glycyrrhiza uralensis , glycyrrhiza , pharmacology , cyp2b6 , drug , traditional medicine , medicine , cyp2c19 , cytochrome p450 , metabolism , alternative medicine , pathology
Although hormone therapy remains the standard of care for women experiencing vasomotor symptoms related to menopause, concerns over an increased risk of stroke and breast cancer have women considering botanical dietary supplements for management of these symptoms. Licorice ( Glycyrrhiza sp .) is popular among women as an alternative for hormone therapy, and it has a long history of use in Traditional Chinese Medicine. To ensure the safe use of licorice dietary supplements, we investigated their potential for drug interactions. Standardized licorice extracts of Glycyrrhiza glabra , G. inflata , and G. uralensis, the three most common species used in dietary supplements, were studied for possible inhibition or induction of cytochrome P450 enzymes involved in drug metabolism. In studies using human liver microsomes, and a cocktail probe substrate assay, all three licorice species showed inhibition of CYP2B6, CYP2C8, CYP2C9 and CYP2C19. G. inflata also inhibited CYP1A2, CYP2D6 and CYP3A4. Induction qualified cryopreserved human hepatocytes were used to predict effects of the extracts on cytochrome P450 enzyme induction. CYP3A4 was unaffected by all Glycyrrhiza sp , and CYP1A2 and CYP2B6 were induced in hepatocytes by G. uralensis and G. inflata . Due to these data, dietary supplements containing G. uralensis and G. inflata are more likely than G. glabra to cause clinically relevant drug interactions. Support or Funding Information National Institutes of Health grant P50 AT000155 from the Office of Dietary Supplements and the National Center for Complementary and Integrative Health and training grant T32 AT007533 from the National Center for Complementary and Integrative Health.