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THE ANTI‐APOPTOTIC EFFECT OF METHANOL LEAF EXTRACT OF AZADIRACHTA INDICA ON LEAD ACETATE‐INDUCED APOPTOSIS IN CARDIAC AND RENAL TISSUES OF MALE WISTAR RATS
Author(s) -
Adeyeoluwa Temitayo Esther,
Oyagbemi Ademola Adetokunbo,
Adedapo Adeolu Alex,
Omobowale Temidayo Olutayo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.667.13
Subject(s) - azadirachta , lead acetate , apoptosis , kidney , chemistry , toxicity , pharmacology , traditional medicine , medicine , endocrinology , biochemistry , organic chemistry
Lead toxicity is an existing and ongoing challenge. The apoptotic effects of lead have been extensively studied and several herbal remedies have been proposed. In this study, the anti‐apoptotic effect of the methanol extract of Azadirachta indica (MEAI) was investigated. 70 adult male Wistar rats were divided into 7 groups. Groups B, C and D were exposed orally to 1.0 mg/ml lead acetate and groups E, F and G exposed to 2.0 mg/ml for 6‐week duration, after which groups C and D were given 100 mg/kg and 200 mg/kg Azadirachta indica methanol leaf extract respectively, groups F and G given 100 mg/kg and 200 mg/kg Azadirachta indica methanol leaf extract respectively, and groups B and E withdrawn from lead treatment for another 6 weeks. Group A, the control group, was given clean drinking water for the duration of 12 weeks. Exposure to 1.0 mg/ml and 2 mg/ml lead (Pb) resulted in an increased expression of caspase 3 and cytochrome C, which were seen to increase with increased concentration of exposure while there was a reduction in the expression of Bcl‐2 in both the cardiac and renal tissues. At 100 mg/Kg treatment, MEAI induced a higher expression of Bcl‐2 in both the cardiac and renal tissues while at 200 mg/kg treatment, there was a reduction in the expressions of Bcl‐2 in the kidney tissues in the 1 mg/ml Lead (Pb) exposed groups. The treatment of rats that were exposed to 2.0 mg/ml Lead (Pb) with 100 mg/kg and 200 mg/kg MEAI, resulted in an increased expression of Bcl‐2 in the kidney tissues but a down regulation in the heart tissues. MEAI at both 100 mg/kg and 200 mg/kg treatment resulted in a reduced expression of cytochrome C in both tissues The results of this study show that at 100 mg/kg and 200 mg/kg concentrations of MEAI, there was an inhibition/reversal of the lead induced apoptosis observed in both the cardiac and renal tissues.