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Naturally, Occurring Neurotrophic Factor from Mu Bie Zi ( Momordica cochinchinensis ) seeds
Author(s) -
Mazzio Elizabeth A,
Georges Beatrice,
Soliman Karam F
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.659.5
Subject(s) - chemistry , nutraceutical , nerve growth factor , chromatography , trypsin , trypsin inhibitor , polyacrylamide gel electrophoresis , biochemistry , food science , enzyme , receptor
The discovery of nerve growth factor (NGF) mimetics could have the potential application for Alzheimer's disease prevention and therapy. Previously, we have conducted a high‐throughput (HTP) screening of over 1100 nutraceutical products (herbs, vitamins, plant‐based phytochemicals, etc.) to determine if any dietary based substance could induce neurite outgrowth similar to 7S NGF at <200ug/mL in PC‐12 cells. Only a single biologically active with compelling NGF mimetic capacity in the seed of Mu Bie Zi , Momordica cochinchinensis (bitter cucumber) (MCS). The objective of the current study is to identify the bioactive substances within the ground seed pit by testing for NGF biological activity in the crude seed solvent extractions and purified proteins. The active protein fraction was subjected to one‐dimensional gel electrophoresis (native), gel staining, sectioning / excision, electro‐elution back into solution and re‐evaluation for NGF mimetic effects. Results obtained show that the NGF mimetic resided in a low abundant visually absent gel section at around 17 kD. The 17kD gel slice was excised, digested and run on a UPLC‐MS/MS –with a Q Exactive Hybrid Quadrupole ‐ Orbitrap Mass Spectrometer. The data were evaluated using X! Tandem, OMS, X Hunter and K‐score algorithms. Proteomic evaluation of the 17kD band was conducted showing the presence of 11S globulin subunit beta, napin, oleosin 18.2 kDa, trypsin inhibitor 1 MCoTI‐I /II (known to contain growth factor cystine knots) and Two Inhibitor Peptide Topologies TIPTOPs (isoforms 1,2,3,5,6), all with taxonomical identities inherent to the cucumber family ( Momordica cochinchinensis, macrophylla, charantia, Cucumis sativus ). Support or Funding Information This research was supported by the National Institute for Minority Health and Health Disparities of the National Institutes of Health through Grant Number G12 MD007582 and Grant Number P20 MD006738