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The Effect of Plumbagin on Proinflammatory Cytokines Expression in LPS‐Activated BV‐2 Microglia Cells
Author(s) -
Messeha Samia S,
Zarmouh Najla O,
Kolta Malak G,
Soliman Karam F.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.657.7
Subject(s) - proinflammatory cytokine , plumbagin , pharmacology , microglia , neuroprotection , nitric oxide synthase , chemistry , nitric oxide , cytokine , inflammation , immunology , biology , genetics , organic chemistry
In the central nervous system, activated microglial cells produce proinflammatory mediators such as inducible nitric oxide (NO2) and cytokines. Uncontrolled release of these mediators can cause neuronal damage in the brain leading to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Therefore, inhibition of these proinflammatory mediators may provide a neuroprotective strategy in preventing neurodegenerative diseases. the other hand, plumbagin is a biologically active compound in the roots of the Indian medicinal plant Plumbago zeylanica L. which has been used in treating rheumatoid arthritis. The present study was conducted to examine the antiinflammatory effect of the plumbagin in LPS‐activated microglial BV‐2 cell model. The obtained data indicated that plumbagin (IC50 = 0.386 uM) was about 20‐fold more potent than l‐N6‐(1‐iminoethyl) lysine (LNL), the selective inhibitor of inducible nitric oxide synthase (iNOS) (IC50 =7.4 uM). Moreover, our immunofluorescence study and ELISA confirmed the positive relationship between NO2 decrease and iNOS expression inhibition. RayBio AAM‐CYT‐3 and 4 cytokine antibody protein arrays were selected for detecting extracellular proinflammatory cytokines expression changes in LPS–activated BV‐2 cells in the presence of plumbagin. In the activated BV‐2 cells, 2 ums of plumbagin significantly inhibited the release of IL‐6, MCP 1, MCP5, MIP‐1 alpha and gamma, IL‐1 alpha, G‐CSF, IL‐12 p40/70 and MDC. The obtained results of ELISA analysis validated the cytokines array data. Taken all together, we concluded that plumbagin is a potent antiinflammatory agent reduces multiple proinflammatory cytokines and iNOS. Support or Funding Information Supported by NIH Grants G12 MD007582 and P20 MD 006738