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Inflammation Response of Phage‐Based Films on Titanium Surface in Vitro
Author(s) -
Sun Yuhua,
Feng Bo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.657.15
Subject(s) - macrophage polarization , secretion , tumor necrosis factor alpha , inflammation , apoptosis , proinflammatory cytokine , immune system , transforming growth factor , phage display , microbiology and biotechnology , chemistry , in vitro , macrophage , biology , immunology , peptide , biochemistry
Phage‐based materials exhibit great potential in the applications of tissue regeneration. However, challenges still exist in the development of this field, such as a good understanding of the immune response to phage‐based biomaterials. The objective of this study was to examine the inflammation response of phage film and phage films chemically grafted peptides in vitro . Three different peptides with different affinity to transforming growth factor β1 (TGF‐β1) were used. Macrophage (RAW 246.7) adhesion, proliferation, apoptosis, polarization and expression levels of pro‐inflammatory cytokines, IFN‐γ, TNF‐α, IL‐6, IL‐1β, and anti‐inflammatory cytokines, IL‐10, IL‐4, TGF‐β1, were evaluated. The results showed that the phage films on Ti surfaces would induce the early apoptosis of the adherent macrophage and reduce the proliferation rate. In late culture period, a small amount of cell aggregation was present, but no foreign body giant cells were found. These films promoted sequential M1 and M2 polarization, as measured by expression of surface markers (CCR7 and CD206) and secretion of cytokines. The third day was the turning point of the polarization with increasing secretion of anti‐inflammatory cytokines, IL‐10 and TGF‐β1, although the ratio of M1 polarization was the greatest on the fifth day. No secretion of IFN‐γ and IL‐4 were detected. Macrophage polarization was primarily co‐regulated by phage films and the secreted factors (IL‐10, TGF‐β1 and TNF‐α). Compared to the control group, phage films downregulated the inflammatory response. In addition, the phage film grafted peptide with greatest affinity to TGF‐β1 induced higher early apoptosis of adherent macrophage and IL‐10 expression. The association with the binding of active TGF‐β1 and adsorption of serum proteins will be further studied in detail. The results of this study provide highly encouraging evidence for the safety of potential application of phage‐based materials in vivo . Support or Funding Information Natural Science Foundation of China (31570955)