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Effect and regulatory mechanisms of low‐density lipoprotein on the growth and migration of A549 human lung cancer cells
Author(s) -
Tsao Shu Ping
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.656.13
Subject(s) - lung cancer , hyperlipidemia , cancer research , cancer , a549 cell , cell growth , viability assay , western blot , cancer cell , cell , mtt assay , cell migration , medicine , biology , endocrinology , biochemistry , gene , diabetes mellitus
Lung cancer is one of the most causative factors of death in cancer. Accumulating evidences implicated the possible effects of hyperlipidemia on increased risk for cancer progression. The purpose of this study was to investigate whether low‐density lipoprotein (LDL), a critical hyperlipidemia effector, has potential roles to modulate the cell growth and migration of lung cancer cells as well as to understand its possible regulatory mechanisms. Cell viability and migration were examined by MTT and wound healing assays, respectively. The translational and transcriptional expressions were monitored by Western blot and RT‐PCR, respectively. Experimental data showed that LDL can significantly increase cell growth and migration of A549 non‐small cell lung cancer cells by activating the ERK1/2‐MAPK pathway. Our study suggested that LDL might play as a stimulator for the progression of lung cancer in the hyperlipidemic condition. Application of lipid lowering agents may be a poteintial remedy for lung cancer therapy.