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Lipidomic profiling of erythocytes in septic patients revels novel biomarkers
Author(s) -
Mecatti Giovana Colozza,
Messias Marcia Cristina Fernandes,
Angolini Célio Fernando Figueiredo,
Oliveira Carvalho Patricia
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.655.6
Subject(s) - sepsis , septic shock , sphingomyelin , pathophysiology , phosphatidylcholine , lipidome , urinalysis , medicine , chemistry , lipidomics , urine , biochemistry , cholesterol , phospholipid , membrane
Sepsis is common and often deadly. It remains the primary cause of death from infection, despite modern medicine. The identification of reliable diagnostic biomarkers for the early detection of this disease is critical and may reduce the mortality rate as it could allow early treatments. The present study deals with the plasma and red cells blood lipidome profiling of patients diagnosed with sepsis and septic shock and aimed to find possible sepsis biomarkers using GC and ESI‐MS (q‐ToF). The data was treated with multivariate data analysis, including principal component analysis (PCA) and (orthogonal) partial least squared discriminant analysis ((O)PLS‐DA). Potential biomarkers including lysophosphatidylcholines (lyso‐PCs) and sphingomyelin (SMs) with specific fatty acid chains were identified. Lyso‐PCs and SMs were down‐regulated whereas saturated and unsaturated phosphatidylcholine (PCs) were up‐regulated in the plasma and erythrocytes of septic patients, which is evidence for deregulated activity of phospholipases. An increase of oleic acid (C18:1 n‐9) accompanied by a decrease in the unsaturation index as well as in the abundances of n‐3 polyunsaturated fatty acids (n‐3 PUFA) was observed in erythrocytes phospholipids patients as compared with healthy controls. This study thus provides new insights into underlying sepsis lipids pathophysiology which may serve clinical diagnosis Support or Funding Information FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo)