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Reduced citrulline flux and concentration in patients with ketosis‐prone diabetes compared to healthy controls and typical type 2 diabetes suggest impaired gut function
Author(s) -
Hsu Jean W,
Mulukutla Surya N,
Bohren Kurt M,
Balasubramanyam Ashok,
Jahoor Farook
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.652.1
Subject(s) - medicine , citrulline , type 2 diabetes , endocrinology , diabetes mellitus , flux (metallurgy) , excretion , diabetic ketoacidosis , chemistry , arginine , biochemistry , organic chemistry , amino acid
A‐β+ Ketosis‐Prone Diabetes (KPD) is a unique subset of type 2 diabetes (T2D) characterized by non‐autoimmune β cell dysfunction causing unprovoked ketoacidosis, followed by partial restoration of insulin secretory function. Production of citrulline occurs almost exclusively in the intestine and in a previous study, we reported significant positive correlations between urinary mannitol excretion and citrulline flux and concentration in healthy adults suggesting that plasma citrulline flux or concentration is a good marker of gut function. The objective of this ongoing study is to assess gut function in KPD, T2D and healthy subjects (12/group) using parameters of citrulline metabolism. Subjects are given 6h primed, constant intravenous infusions of 2 H 2 ‐Cit after an overnight fast to measure citrulline flux. Preliminary results are shown in the table. Our preliminary results show that citrulline flux and concentration were lower in patients with KPD compared to controls and “usual” T2D. These results suggest that patients with KPD have reduced gut function. Support or Funding Information Supported by NIH Grant # 5R01DK101411 Citrulline flux and concentration in control subjects, T2D and KPD patientsControls T2D KPD P‐ValueCitrulline Flux μmol/kg LBM/h 9.23 ± 0.71 a 10.1 ± 1.01 a 5.64 ± 0.33 b 0.0121Citrulline Concentration μmol/L 27.2 ± 1.31 a 21.6 ± 2.21 a 12.4 ± 3.02 b 0.0002Means with different superscript letters are significantly different, P < 0.05 (post‐hoc Tukey's multiple comparison test).