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Hepatoprotective Effect of Centella asiatica Extract (ECa233) in Rotenone‐induced Liver Injury Rats
Author(s) -
Tansawat Rossarin,
Intararuchikul Thidarat,
Rodsiri Ratchanee,
Meksawan Kulwara,
Warisnoicharoen Warangkana
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.647.10
Subject(s) - rotenone , centella , tbars , pharmacology , catalase , superoxide dismutase , liver injury , glutathione peroxidase , antioxidant , thiobarbituric acid , hepatoprotection , chemistry , traditional medicine , glutathione , lipid peroxidation , biochemistry , biology , medicine , enzyme , mitochondrion
Liveris a vital organ responsible for many essential functions related to digestion, metabolism and detoxification. Excessive toxins can damage the liver cells, leading to liver diseases. Hepatoprotective activities from plant extracts have been investigated by a number of researches worldwide. Though some previous studies have revealed the liver protective effects of Centella asiatica , the component of this herb were varied among the studies. Thus, the standardized extract of Centella asiatica (ECa233) is established for quality control of the major compounds. ECa233 contains > 80% triterpenoids, and the ratio of madecassoside to asiaticoside is 3:2. The aim of this study was to investigate hepatoprotective effects of ECa233 against rotenone in rotenone‐induced liver injury rats. Male Wistar rats were randomly divided into 3 groups (N = 18); Control, Rotenone (positive control) and ECa. Ratsin ECa treated group received 10 mg/kg of ECa233 (p.o.) for 20 days, followed by rotenone (2.5 mg/kg) i.p. injection to induce toxicity before sacrificed. Blood samples were collected for hepatic enzyme tests. Liver tissues were used for antioxidanttests including thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) assays. The results showed that rotenone treated group had elevated AST and ALT enzymes. TBARS levels of the ECa group were significantly lowered than the positive control (P< 0.05). For the antioxidant capacity tests, ECa group had higher SOD levels as compared to the rotenone group (P < 0.05). CAT activity of rotenone group was statistically significant decreased compared to control (P < 0.05) while CAT levels of the ECa were not different from the control group, but significant higher than rotenone treated group (P < 0.01). No significant difference in GPx levels was found among the three treatments. In summary, it can be pointedout that ECa233 has hepatoprotective activities against rotenone toxicity by decreasingoxidative stress biomarker (TBARS) and increasing antioxidant enzymes SOD and CAT in the liver. This new findings might establish novel evidence base to create value‐added of Centella asiatica as a dietary supplements or medicines for liver disorders in the future.