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Cornus officinalis Polyphenols Reduce Senescence of Vascular Cells by Modulating the Antioxidant Response
Author(s) -
Salazar Gloria,
Morgan Hannah,
Feresin Rafaela G,
Klarich Dawn Kylee S
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.646.59
Subject(s) - oxidative stress , reactive oxygen species , senescence , antioxidant , vascular smooth muscle , downregulation and upregulation , chemistry , sod2 , mitochondrion , nadph oxidase , biochemistry , microbiology and biotechnology , superoxide dismutase , biology , endocrinology , smooth muscle , gene
Physiological changes observed during aging, like accumulation of senescent cells, are strongly associated with the development of cardiovascular disease (CVD). Senescent cells secrete reactive oxygen species (ROS), inflammatory molecules and extracellular matrix components causing inflammation and oxidative stress that accelerate tissue dysfunction during aging. The purpose of this study was to investigate whether the cornus officinalis (CO) berry, used in Traditional Chinese Medicine can protect vascular cells from cellular senescence and the molecular mechanism involved. We used vascular smooth muscle cells (VSMCs) treated with angiotensin II (Ang II), a strong stimulator of oxidative stress and senescence, as a model. VSMCs were treated with and without Ang II and CO polyphenol extract for measurements of ROS levels (cytosol and mitochondria), senescence, and expression of antioxidant enzymes as well as NADPH oxidases (Nox). We found that 50–100 microg/ml CO polyphenol extract strongly downregulated the increase in ROS levels and senescence induced by Ang II. This effect was associated with the upregulation of the antioxidant enzymes SOD1, SOD2 and catalase and the downregulation of Nox1. Further, CO polyphenol extract reduced mitochondrial ROS, suggesting that polyphenol compounds in this berry may target the two major sources of ROS, Nox and mitochondria, to reduce senescence of VSMCs. In conclusion, our data suggest that CO polyphenols may slowdown the progression of vascular aging and the incidence of CVD. Support or Funding Information American Heart Association (14GRNT20180028) and the Florida State University Council on Research and Creativity (COFRS)