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Effect of resveratrol in methylation promoter of FNDC5 gene in skeletal muscle and adipose tissue
Author(s) -
HerediaLugo Victor,
GarciaTobilla Pilar,
Rodriguez Mauricio,
Palacios Berenice
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.646.55
Subject(s) - resveratrol , dna methylation , skeletal muscle , fndc5 , epigenetics , adipose tissue , methylation , white adipose tissue , promoter , gene , gene expression , biology , microbiology and biotechnology , endocrinology , medicine , chemistry , genetics , biochemistry , cell , fibronectin
Irisin has been associated with the beneficial effects of exercise training. The study of epigenetics, it has been shown that several responses in gene expression are controlled by DNA methylation. The aim of this study was to identify the effect of resveratrol consumption in FNDC5 promoter methylation in skeletal muscle and adiposse tisue. The experimental animals were randomly assigned to 2 experimental diets; 1. Chow diet (CON) and 2. Resveratrol at 0.1% (RSV). Subsequently, white adipose tissue and skeletal muscle samples were obtained for genomic DNA extraction, cloning of the promoter region and sequencing of the library was performed. Our results, showed that resveratrol was able to increase the expression of FNDC5 in skeletal muscle in mice. No effect was observed in methylation patterns in either tissues. According to the results obtained in this study, resveratrol does not regulate the FNDC5 gene by DNA methylation.