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The synergistic anti‐obesity effect of Gelidium elegans extract and orlistat in vivo and in vitro
Author(s) -
Choi SeoYun,
Park MinKi,
Choi Jia,
Koh EunJeong,
Seo YoungJin,
Song JiHyeon,
Chei Sungwoo,
Hwang JiHyun,
Lee YeonJoo,
Lee BooYong
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.646.10
Subject(s) - orlistat , adipocyte , in vivo , adipogenesis , chemistry , anti obesity , obesity , food science , caenorhabditis elegans , pharmacology , biology , biochemistry , endocrinology , in vitro , weight loss , adipose tissue , microbiology and biotechnology , gene
Obesity is caused by imbalanced food intake and physical activity. A plethora of pharmaceutical agents exist to reduce body weight and associated symptoms in obese patients. However, these medications are not without adverse effects. Therefore, a natural, non‐toxic and cost‐effective food ingredient, such as Gelidium elegans , warrants further consideration in the anti‐obesity arena. Gelidium elegans is a species of red algae that mostly grows in the northeastern Sea of Asia. In this study, we investigated the molecular mechanism of adipogenesis inhibition induced by the ethanol extract of Gelidium elegans on 3T3‐L1 adipocytes and high fat diet mice. We found that exposure to 12.5 and 25 μg/mL concentrations of the Gelidium elegans ethanol extract inhibited adipocyte differentiation through the regulation of PPARγ, C/EBPα, and FABP4 expression. Moreover, we found that exposure to the 25 mg/mL concentration of the Gelidium elegans ethanol extract caused a three‐fold increase in the expression of genes related to brown adipocyte differentiation (such as UCP‐1 and PRDM16) compared with the control group. To elucidate the synergistic anti‐obesity effects of Gelidium elegans , male ICR mice were fed on high fat diets and with or without Gelidium elegans extract (50mg/kg and 200mg/kg) and orlistat (20mg/kg) intervention for 8 weeks. The body weight increase and weight significantly reduced by the diets containing Gelidium elegans extract (200mg/kg) and orlistat + Gelidium elegans extract (50mg/kg). Serum concentration of triglycerides (TG) and glucose were decreased by Gelidium elegans extract and orlistat. Also, we found that Gelidium elegans (200mg/kg/day) prevented hyperglycemia. Our findings suggest that Gelidium elegans ethanol extract enhances energy expenditure and suppresses adipocyte differentiation. Gelidium elegans ethanol extract may be a safe, effective and economical food grade ingredient for obese patients.