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The Effects of Ipriflavone, Isoflavone, and 17b‐Estradiol on Body Composition in Hamster Models of Ovariectomy
Author(s) -
Foley Elizabeth M,
Pourafshar Shirin,
Navaei Negin,
Akhavan Neda S,
George Kelli S,
Arjmandi Bahram H
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.645.17
Subject(s) - ovariectomized rat , endocrinology , medicine , hamster , body weight , mesocricetus , isoflavones , soy protein , syrian hamsters , chemistry , hormone , food science
Soy isoflavones have been found to have favorable effects on body composition in postmenopausal women. Ipriflavone is a synthetic isoflavone theorized to behave similarly to natural isoflavones. The purpose of this study was to compare and contrast the effects of three different treatments: isoflavone (ISO), ipriflavone (IP), and 17β‐estradiol (E 2 ) on body composition in ovariectomized (Ovx) hamsters, and to evaluate the hamster's response to ovarian hormone deficiency. For this purpose, we used sixty‐five female Golden Syrian Hamsters, which were divided into five groups of twelve hamsters per group. All groups were Ovx except the sham‐operated (Sham) group. Thus, the groups included Sham, Ovx, Ovx+IP, Ovx+E 2 , and Ovx+ISO. All groups received an isoflavone‐depleted soy‐based diet, a reduced isoflavone diet (containing less than 10% of the amount usually found in soy protein isolate) except for the ISO group, which received a soy diet containing normal levels of isoflavone (2.3mg/g protein). Ipriflavone was given by gavaging (200mg/kg body weight/day) and subcutaneous injections of E 2 (10mg/kg body weight) were administered on a daily basis. After 65 days of treatment, animals were exsanguinated and the bones and organs were collected for analyses. Ovariectomy was confirmed by uterine weight (0.129 ± 0.014, P < 0.0001). Hamsters behaved similarly to rats in that Ovx hamsters gained excess body fat irrespective of pair feeding. As expected, abdominal fat in Ovx hamsters in E 2 and ISO was reduced significantly by 1.93 ± 0.24, and 1.44 ± 0.19, P <0.0001, respectively. Additionally, relative abdominal fat was increased in Ovx (2.47 ± 0.24, P <0.0001), but it was decreased in both E 2 (1.54 ± 0.18, P <0.0001) and ISO (1.19 ± 0.15, P <0.0001) groups. The findings of this study indicate that isoflavones act similarly to estrogen in a hamster model of postmenopausal hormone deficiency, and can improve body composition thus decreasing the risk of other chronic diseases.