Premium
Correlation of mRNA Levels with CpG Methylation Status of Genes Regulated by Fatty Acids in Differentiated Adipocytes
Author(s) -
Li Yi,
Cheng Elliott,
Bower Brittney
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.644.17
Subject(s) - dna methylation , methylation , gene , epigenetics , cpg site , bisulfite sequencing , biology , adipose tissue , polyunsaturated fatty acid , differentially methylated regions , adipocyte , gene expression , biochemistry , microbiology and biotechnology , fatty acid
Adipose and muscle tissues are significantly affected in obesity and type 2 diabetes. In order to study the involvement of epigenetic modifications in mediating gene expression induced by diets, 3T3‐L1 preadipocytes were cultured in media containing saturated fatty acids to mimic hyperlipidemia conditions, which occur during the development of obesity and type 2 diabetes. DNA methylation sequencing was used to identify the methylated regions from the DNA samples isolated from the differentiated 3T3‐L1 adipocytes treated with saturated and polyunsaturated fatty acids. In combination with the pool of previously identified genes that mRNA levels were regulated by fatty acids, we have found correlation between some of the highly methylated regions with the genes that were regulated by fatty acids. We are currently individually validating methylation status of these highly methylated genes including RASA2, MEF2C, and GRP78. We are also studying the signaling pathways mediating methylation of these genes. Support or Funding Information This research is supported by grant SC3GM116737 from the NIGMS of the NIH to Y. Li.