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Heart Inflammation Is Associated to Carbohydrate‐Enriched Diet in an Experimental Model of Obesity
Author(s) -
Ferreira Ana Lucia Anjos,
Ferron Artur J. T.,
Moreto Fernando,
Francisqueti Fabiane V.,
Kitawara Koody A. H.,
Lo Angelo T. C.,
Ferraz Ana Paula C. R.,
Garcia Jessica L.,
Ghiraldeli Luciana,
Correa Camila R.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.643.11
Subject(s) - adipose tissue , medicine , endocrinology , fructose , tumor necrosis factor alpha , cytokine , inflammation , carbohydrate , ventricle , monocyte , chemistry , biochemistry
The study aimed to verify the effects of 30‐wk period of carbohydrate‐enriched diet (CHO) on markers of inflammation in heart tissue. Young (8wk‐old) male Wistar rats were randomized into control (Co, n=6) or obese (OB, n=6) groups. Co group was feed with a standard normocaloric chow and water whereas OB group was feed with the hypercaloric CHO diet, which provided 78% of total energy from fructose (in chow) and sucrose (in chow and water). After 30‐wk treatment, animals were euthanized and adipose and heart tissues were collected. Adiposity index was obtained by the sum of visceral, epididymal and retro‐peritoneal adipose tissue mass adjusted by the remaining body mass. Heart was immediately excised and an aliquot of left ventricle (LV) was snap‐frozen in liquid nitrogen and stored −80°C until analyses. Inflammatory cytokines monocyte chemoattractant protein 1 (MCP‐1), interleukin 6 (IL‐6) and tumor necrosis factor alfa (TNF‐α) was measured in LV tissue homogenates by specific ELISA Duoset® kit (R&D Systems). Cytokine levels were adjusted by total protein content in LV homogenates. T ‐test was used to compare groups and significance tolerance was set as p<0.05. After 30‐wk of CHO diet, adiposity index was markedly higher in OB group characterizing obesity development. Also, LV from OB group expressed higher levels of MCP‐1 (Co: 14.0±2.5pg/mL; OB: 32.7±5.8pg/mL), IL‐6 (Co: 23.7±3.5pg/mL; OB: 44.7±11.9pg/mL) and TNF‐α (Co: 78.3±11.8pg/mL; OB: 107.8±18.9pg/mL). These results indicate that a 30‐wk CHO diet is able to induce obesity and heart tissue inflammation which can result in cardiac dysfunction. Support or Funding Information Supported by FAPESP.CHO‐diet effects on adiposity index and inflammatory cytokines.