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Protein Carbonylation Is Related to Liver Damage in an Experimental Model of Obesity
Author(s) -
Moreto Fernando,
Ferron Artur,
Francisqueti Fabiane,
Hasimoto Fabiana,
Garcia Jessica,
Ghiraldeli Luciana,
Ribeiro Elisabeth,
Correa Camila,
Ferreira Ana Lucia
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.643.10
Subject(s) - medicine , endocrinology , fructose , chemistry , alanine aminotransferase , venous blood , obesity , biochemistry
The present study verified the effects of 20‐wk period of carbohydrate‐enriched (CHO) diet on plasma metabolic and liver damage biomarkers and protein carbonyl (PC) levels. Young (8wk‐old) male Wistar rats were randomized into control (Co, n=6) or obese (Ob, n=6) groups. Co group was feed with a standard normocaloric chow and water whereas Ob group was feed with the hypercaloric CHO diet, which provided 78% of total energy from fructose (in chow) and sucrose (in chow and water). After 20‐wk treatment body weight (BW) was recorded and venous blood was drawn (after 12h‐fasting) from tail in order to evaluate the diet's efficiency on inducing obesity with metabolic dysfunctions. It was measured fasting blood glucose (FBG), plasma triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and PC levels by the alkaline method. AST/ALT ratio was used as liver damage biomarker. Statistical analysis used t‐test, Pearson correlation and multiple regression with a significance tolerance of 5%. Compared to Co, Ob group showed higher BW, FBG, TG and plasma PC after 20wk‐CHO diet. AST/ALT ratio was not different between groups. Clustering all animals, it was observed that plasma PC was direct related to AST/ALT ratio (r=0.74; p=0.006) and tended to be related to FBG (r=0.48; p=0.05). Multiple regression analysis model showed that BW, FBG, TG and AST/ALT ratio represented a significant model (p=0.04) with 55% of influence (adjusted R 2 =0.55) on plasma PC including AST/ALT ratio (Beta=0.677±0.22; p=0.02) that was the major influencing factor. In conclusion, a 20‐wk CHO diet efficiently induced metabolic alterations and higher plasma PC levels. The high protein carbonylation status seems to be involved with liver damaging processes. Support or Funding Information Supported by FAPESP.Plasma markers of Co and Ob groups.PC levels correlations and multiple regression