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A possible emerging role of Stachys siedlbodii MIQ extract in neuroprotection: in vitro and in vivo
Author(s) -
Ravichandran Vijaya Abinaya,
Kim Mina,
Cha Youn Soo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.636.26
Subject(s) - cholinergic , neuroprotection , in vivo , cholinergic neuron , oxidative stress , pharmacology , acetylcholine , hippocampus , choline , memory improvement , chemistry , medicine , neuroscience , psychology , biology , biochemistry , cognition , microbiology and biotechnology
Dementia is the most common problem affecting older people; recently demonstrated the importance of oxidative process and loss of cholinergic neuron in parts of the brain subserving aspects of memory. Plants are an important source of essential nutrients, such as choline, vitamins, phenyl ethanoid glycosides and saponins, etc. These nutrients have shown promising effect to prevent or delay the onset of neurological disorder. This work aims to evaluate the potential effect of Stachys siedlbodii MIQ (SS) against oxidative stress induced by hydrogen peroxide (H 2 O 2 ) in SK‐N‐SH cells, and memory enhancement in ICR mice using the passive avoidance test. This study also investigated the role of cholinergic activity in SS induced brain tissue. In SK‐N‐SH cell culture, SS could partially antagonize the effects of 150 μM H 2 O 2 on cell viability. In vivo study, SS (100 mg/kg, 250 mg/kg, and 500 mg/kg) was administered orally to mice for 4 weeks. Treatment with SS, based on dose dependent manner had a significantly longer step‐through latency time than the control suggesting retained of fear memory. SS improved cholinergic system reactivity by increased level of acetylcholine in hippocampus and frontal cortex. These results suggest that SS administration significantly ameliorate the memory retention, by improving cholinergic activity on mice brain. Support or Funding Information This research was financially supported by the Ministry of Trade, Industry, and Energy (MOTIE), Korea, under the “Regional Specialized Industry Development Program” supervised by the Korea Institute for Advancement of Technology (KIAT)