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Fis1 activity in pre‐ and post‐assembly of the yeast mitochondrial fission machinery.
Author(s) -
Harwig Megan Cleland,
Hill R. Blake
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.634.8
Subject(s) - fis1 , mitochondrial fission , fission , mitochondrial fusion , mitochondrion , microbiology and biotechnology , yeast , biology , mitochondrial dna , genetics , gene , physics , nuclear physics , neutron
Far from static organelles, mitochondria frequently undergo fusion and fission events to help maintain a healthy mitochondrial network. These processes are vital for a variety of cellular tasks including maintenance of the mitochondrial genome as well as the mitochondrial membrane potential. In yeast, a mitochondrial outer membrane fission protein 1 (Fis1) is essential for mitochondrial fission; however, the precise role of Fis1 has been a matter of debate. Fis1 is highly conserved and interacts with two cytosolic fission proteins, Mdv1 and Dnm1 to possibly recruit them to sites of scission, however, the details of these interactions are not well understood. We recently demonstrated that mutating a key residue (E78) of Fis1 helped demonstrate a post‐recruitment role for Fis1. Here, our analyses with additional point mutations of Fis1 points to a revised model for Fis1 activity in yeast mitochondrial fission. Support or Funding Information NIH R01 GM067180