Premium
Characterizing of the Novel Gene At4g33666 in the Abiotic Stress Response in Arabidopsis
Author(s) -
Clark Amber Elizabeth Miller,
Wershoven Nicole,
Palmer Christine
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.617.6
Subject(s) - abiotic stress , abiotic component , arabidopsis , arabidopsis thaliana , gene , biology , computational biology , gene expression , osmotic shock , genetics , ecology , mutant
Expanding populations and environmental changes can leave the plants we depend upon to meet our food and energy needs facing increasingly difficult growing conditions. Enhancing our understanding of the methods that plants naturally employ to overcome abiotic factors such as heat, drought, high salinity, or poor light will enable us to better select for plants that can succeed under challenging conditions. Here we examine At4g33666 , a novel gene in Arabidopsis thaliana , which is thought to play a role in perceiving or overcoming abiotic stressors. In order to begin to understand the role of this gene in the stress response, we have examined expression at both an RNA and protein level. We extracted RNA from plants exposed to abiotic stress and performed quantitative PCR. To visualize phenotypic changes caused by the loss or overexpression of the gene, we measured the rate of germination of different genotypes under osmotic stress. This protein is hypothesized to be localized to the chloroplast, and to learn more about post translational modifications that might occur, we used immunoprecipitation to isolate protein for analysis via mass spectrometry. By studying the role of At4g33666 , we can improve our understanding of plant stress responses and optimize success of the plants we depend upon. Support or Funding Information This research was supported by a Castleton University student research grant.