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Expression of E3‐Ubiquitin Ligase genes in P. patens during sexual reproduction
Author(s) -
Abrash Elizabeth Whitney,
Hicks Karen A
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.617.3
Subject(s) - ubiquitin ligase , biology , ubiquitin , sexual reproduction , gene , functional divergence , genetics , microbiology and biotechnology , gene expression , gene family
Bryophytes and flowering plants diverged from a common ancestor 450 million years ago, yet both are induced to sexual reproduction as a result of seasonal cues such as day length and ambient temperature. While these pathways have been studied well in flowering plants, it is unknown whether this mechanism evolved before or after the divergence of bryophytes. In order to gain insight into the evolution of these pathways, our lab studies the moss Physcomitrella patens. Here we study the expression of two E3‐Ubiquitin‐Ligase genes that are differentially expressed in response to temperature cues based on RNA Sequencing analysis. E3‐Ubiquitin‐Ligases are part of the process known as ubiquitination, in which ubiquitin is added to a protein leading to degradation. Ubiquitin‐Ligase proteins have been shown to regulate photoperiod and temperature signaling in flowering plants. We hypothesize that regulation of sexual reproduction evolved prior to the divergence of bryophytes, and as such E3‐Ubiquitin‐Ligases will play a similar role in P. patens to that in flowering plants. These results will confirm RNA Seq analysis and identify potential knock out genes for future study.